Journal: International Journal of Medical Sciences

Article Title: Ginsenoside Rg1 alleviates acute liver injury through the induction of autophagy and suppressing NF-κB/NLRP3 inflammasome signaling pathway

doi: 10.7150/ijms.50919

Figure Lengend Snippet: G-Rg1 suppressed apoptosis in mice with CCl 4 -induced acute liver injury through autophagic induction. (A) Apoptotic cells were stained with a TUNEL apoptosis detection kit in control group, G-Rg1(4 mg/ml) control group, G-Rg1(4 mg/ml) + RPA(1 mg/kg) group and G-Rg1(4 mg/ml) + 3-MA(1 mg/kg) group at 24 h after the CCl 4 injection. All of the images were obtained on an inverted fluorescence microscope. (B) Flow cytometry was also performed to detect the apoptotic hepatocytes (n=3). *P<0.05.

Article Snippet: All images were obtained with an inverted fluorescence microscope (Nikon Eclipse E800, Tokyo, Japan).

Techniques: Staining, TUNEL Assay, Control, Injection, Fluorescence, Microscopy, Flow Cytometry

Journal: PLoS ONE

Article Title: Deregulation of Mitochondria-Shaping Proteins Opa-1 and Drp-1 in Manganese-Induced Apoptosis

doi: 10.1371/journal.pone.0091848

Figure Lengend Snippet: Mitochondria were visualized with MitoTracker Red CMXRos (75 nM) under a fluorescence microscope. Representative images acquired and deconvoluted are shown . Scale bar: 10 µm. Three categories of cells exhibiting different mitochondrial morphology were scored: tubular mitochondria (normal), intermediate mitochondria (filamentous with swelling regions) and fragmented (globular). Cells classified as “loss of Δψm” refers to those exhibiting nuclear condensation and Δψm collapse ( A ); 200 cells/sample were counted and classified according to item A ( B ). Two independent experiments were conducted. Statistical significance, ***p<0.001 vs. control and ##p<0.01 vs. Mn.

Article Snippet: Samples were examined under a confocal fluorescence microscope Nikon Eclipse E800 C1 (Nikon Instech Co., Ltd., Karagawa, Japan), equipped with objective lens Nikon Plan Apo 60X/1.40 Oil (Mitotracker Red: λ ex : 544 nm; λ em : 570-LP nm; Alexa 488: λ ex : 488 nm; λ em : 515–530 nm).

Techniques: Fluorescence, Microscopy, Control